Development of conditions for Pyrazidol identification in the urine in the presence of its biotransformation products by thin layer chromatography and mass spectrometry

For the analytical diagnosis of drug poisoning, it is important to develop conditions for detection both native compounds and products their biotransformation in biological samples. Aim. To a method isolating antidepressant Pyrazidol from human urine presence its determine that are suitable diagnostics thymoleptic intoxication by thin layer chromatography. Materials methods. The study was conducted with samples collected after taking single therapeutic dose Pyrazidol. subjected acid hydrolysis, metabolites were extracted hydrolysate chloroform an alkaline medium at pH 8-9. Concomitant endogenous admixtures removed extraction diethyl ether acidic 1. chromatographic extracts, four mobile phases recommended International Association Forensic Toxicologists TLC screening drugs, Merck plates used. Color reactions performed on pieces number chromogenic reagents most commonly used chemico-toxicological analysis. Metabolites identified electron impact mass spectrometry. Results discussion. substance dehydropyrazidol detected hydrolysates TLC, mobility parameters systems, as well results color determined. Conclusions. Conditions product have been proposed. detecting compound extracts spectrometry has developed. use practice forensic clinical toxicology.